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Cell Theory and Parts of a Microscope Worksheet with Answers (Grade 8 Science)

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📄 Cell Theory and Parts of a Microscope Worksheet with Answers (Grade 8 Science)

A free printable microscope and cell theory worksheet for Grade 8 science.

By Kris Reddy, Ontario teacher · Updated October 17, 2026 · 37 questions (30 marks) · PDF + answer key

This free parts of a microscope worksheet combines the compound light microscope with cell theory for Grade 8 science. Students label 12 parts of a detailed microscope diagram, match parts to their jobs, calculate total magnification, and put the steps for focusing a slide in the right order.

The second page covers field of view and estimating the size of a cell, why the letter "e" looks upside down and backwards, the three parts of the cell theory, the scientists who built it (Hooke, van Leeuwenhoek, Schwann and Virchow), and a microscope-safety mistake. Every calculation was checked by computer. To print it, use the Download button above.

  • Grade 8 Science
  • 30 marks
  • 40–50 minutes
  • Labelled answer key

What's in This Parts of a Microscope Worksheet

PartSkill practisedMarks
ALabel 12 parts of a compound light microscope6
BTotal magnification3
CWhat does each part do?3
DOrder the steps for focusing3
EField of view and cell size6
FWhat you see (inverted image)2
GThe cell theory3
HWho discovered what?2
ISpot the mistake2

The parts of a microscope worksheet supports the Ontario Grade 8 science curriculum, Life Systems: Cells, where students learn the cell theory and use a microscope to observe cells safely. It pairs well with the cell organelles worksheet and the plant and animal cell worksheet.

Parts of a Compound Light Microscope

A compound light microscope uses two sets of lenses, the eyepiece and an objective lens, to magnify a specimen. Light from below passes up through a thin slide, through the lenses, and into your eye. Here is the diagram students label in Part A of the parts of a microscope worksheet:

123456789101112
1 arm · 2 coarse adjustment knob · 3 fine adjustment knob · 4 base · 5 eyepiece · 6 body tube · 7 revolving nosepiece · 8 objective lens · 9 stage clips · 10 stage · 11 diaphragm · 12 light source
PartWhat it does
Eyepiece (ocular)The lens you look through; usually 10×
Body tubeHolds the eyepiece at the right distance from the objectives
Revolving nosepieceRotates to switch between objective lenses
Objective lensesMain magnifying lenses, usually 4×, 10× and 40×
Stage and stage clipsFlat platform that holds the slide; clips keep it in place
DiaphragmControls how much light reaches the specimen
Light sourceShines light up through the specimen
Coarse adjustment knobLarge focusing movements, on low power only
Fine adjustment knobSmall, sharp focusing; the only knob to use on high power
Arm and baseHold up the microscope; carry it by the arm with a hand under the base

Part C of the parts of a microscope worksheet checks these jobs. Always carry a microscope with two hands: one on the arm and one under the base. For more background, see optical microscope on Wikipedia.

Magnification and Field of View

To find the total magnification, multiply the eyepiece by the objective lens: 10× × 40× = 400×. The field of view is the diameter of the bright circle you see. As magnification goes up, the field of view gets smaller, by the same factor:

EyepieceObjectiveTotal magnificationField of view
10×4×40×4.5 mm
10×10×100×1.8 mm
10×40×400×0.45 mm

Field of view lets you estimate cell size. If 6 onion cells fit across a 1.8 mm field at 100×, one cell is about 1.8 ÷ 6 = 0.3 mm, or 300 µm (micrometres). Part E of the parts of a microscope worksheet walks through these steps. Very small sizes like these are often written in scientific notation; see the scientific notation worksheet.

Why the Image Is Upside Down

The lenses in a compound microscope invert the image. A letter e on a slide looks upside down and backwards, as if it had been turned 180° (option D below). It also means that if you move the slide to the left, the image moves to the right. Part F of the parts of a microscope worksheet tests both ideas.

eA
eB
eC
eD

The Cell Theory

Microscopes made the cell theory possible. It has three parts:

  1. All living things are made of one or more cells.
  2. The cell is the basic unit of structure and function in living things.
  3. All cells come from pre-existing cells.

It took almost 200 years and several scientists to piece this together:

YearScientistContribution
1665Robert HookeLooked at cork and named the tiny boxes "cells"
1670sAntonie van LeeuwenhoekFirst to see living microorganisms
1838Matthias SchleidenAll plants are made of cells
1839Theodor SchwannAll animals are made of cells
1855Rudolf VirchowAll cells come from other cells

Part H of the parts of a microscope worksheet asks students to match four of these scientists to their discoveries. Read more about the cell theory on Wikipedia.

Parts of a Microscope Worksheet Practice Questions

These questions match the parts of a microscope worksheet PDF, which has the full microscope diagram and the letter-e options drawn.

Part A – Label the Microscope

Label 12 parts (questions 1–12) using the word bank.

Part B – Magnification

  1. 10× eyepiece and 4× objective
  2. 10× eyepiece and 10× objective
  3. 10× eyepiece and 40× objective
  4. 400× total, 40× objective → eyepiece: find the missing value
  5. 600× total, 15× eyepiece → objective: find the missing value

Part C – What Does Each Part Do?

  1. Holds the slide in place on the stage
  2. Rotates to change from one objective lens to another
  3. Controls how much light passes up through the specimen
  4. Moves the stage a lot, for focusing on low power only
  5. Makes small, sharp focus changes (use on high power)
  6. The lens you look through; it usually magnifies 10×

Part D – Focusing a Microscope

  1. Put five mixed-up focusing steps in the correct order.

Part E – Field of View and Cell Size

  1. At 40× the field of view is 4.5 mm across. What is the field of view at 100×?
  2. At 100×, about 6 onion cells fit end to end across the field of view. Estimate the length of one onion cell in mm and in micrometres (1 mm = 1000 µm).
  3. What is the field of view at 400×? Give your answer in mm and µm. Why do you see less of the specimen?

Part F – What You See

  1. Which drawing shows how the letter e looks through the microscope?
  2. You move the slide to the left on the stage. Which way does the image move as you look through the eyepiece?

Part G – The Cell Theory

  1. All living things are made of one or more ________.
  2. The cell is the basic unit of ________ and function in living things.
  3. All cells come from ________ cells.

Part H – Who Discovered What?

  1. Looked at thin slices of cork in 1665 and named the tiny boxes "cells"
  2. Built simple microscopes and was the first to see living microorganisms (1670s)
  3. Concluded that all animals are made of cells (1839)
  4. Stated that all cells come from other cells (1855)

Part I – Spot the Mistake

  1. A student focuses on high power (400×) using the coarse adjustment knob. Explain why this is a problem.

Parts of a Microscope Worksheet Answer Key

Open each part to check the parts of a microscope worksheet answers. The printable key, with the labelled diagram, is on the last two pages of the PDF.

Part A answers (Q1–12)
  1. arm
  2. coarse adjustment knob
  3. fine adjustment knob
  4. base
  5. eyepiece
  6. body tube
  7. revolving nosepiece
  8. objective lens
  9. stage clips
  10. stage
  11. diaphragm
  12. light source
Part B answers (Q13–17)
  1. 40×
  2. 100×
  3. 400×
  4. 10×
  5. 40×
Part C answers (Q18–23)
  1. stage clips
  2. revolving nosepiece
  3. diaphragm
  4. coarse adjustment knob
  5. fine adjustment knob
  6. eyepiece
Part D answer (Q24)
  1. 1. Turn the revolving nosepiece to the lowest-power (shortest) objective.; 2. Place the slide on the stage and hold it with the stage clips.; 3. Look through the eyepiece and turn the coarse adjustment knob to focus.; 4. Use the fine adjustment knob to make the image sharp.; 5. Switch to a higher-power objective and focus with the fine adjustment knob only.
Part E answers (Q25–27)
  1. FOV gets smaller as magnification gets bigger: 4.5 × 40 ÷ 100 = 1.8 mm
  2. 1.8 mm ÷ 6 = 0.3 mm = 300 µm
  3. 4.5 × 40 ÷ 400 = 0.45 mm = 450 µm. Higher power zooms in on a smaller area.
Part F answers (Q28–29)
  1. D: upside down and backwards (turned 180°)
  2. To the right. The image is inverted, so it moves the opposite way.
Part G answers (Q30–32)
  1. cells
  2. structure
  3. pre-existing (other)
Part H answers (Q33–36)
  1. Robert Hooke
  2. Antonie van Leeuwenhoek
  3. Theodor Schwann
  4. Rudolf Virchow
Part I answer (Q37)
  1. On high power the objective is very close to the slide. The coarse knob can crash the lens into the slide and break it. Use only the fine adjustment knob.

5 Common Microscope Mistakes

These come up often in class and when marking this parts of a microscope worksheet.

  1. Using the coarse knob on high power. It can crash the objective into the slide.
  2. Starting on high power. Always find the specimen on the lowest objective first.
  3. Adding instead of multiplying. 10× and 40× give 400×, not 50×.
  4. Forgetting the image is inverted. Move the slide the opposite way to the one you want.
  5. Mixing up mm and µm. 1 mm = 1000 µm.

How to Use This Parts of a Microscope Worksheet in Class

  • Lesson 1: Parts A–D of the parts of a microscope worksheet before students use real microscopes for the first time.
  • Lesson 2: Parts E–I, then measure the field of view with a clear ruler and estimate the size of onion cells.
  • Extension: Research how electron microscopes reach 100 000× or more.

For more practice, try the scientific method quiz and the biological kingdoms quiz.

Frequently Asked Questions

Is this parts of a microscope worksheet free?

Yes. The worksheet and its answer key are free to download and print as one PDF, with no account needed.

What are the main parts of a compound light microscope?

The eyepiece, body tube, revolving nosepiece, objective lenses, stage, stage clips, diaphragm, light source, coarse and fine adjustment knobs, arm and base.

How do you calculate total magnification?

Multiply the eyepiece magnification by the objective lens magnification. A 10× eyepiece with a 40× objective gives 10 × 40 = 400×.

What are the three parts of the cell theory?

All living things are made of one or more cells; the cell is the basic unit of structure and function in living things; and all cells come from pre-existing cells.

Why does an image look upside down under a microscope?

The lenses of a compound microscope invert the image, so it appears upside down and backwards. Moving the slide left makes the image move right.

More Free Grade 8 Science Resources

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